Lumbar puncture (LP) and analysis of cerebrospinal fluid (CSF) has been part of the medical profession for over one hundred years. This relatively simple procedure is widely employed, yet a surprising number of physicians are poorly educated in regards to when and how this procedure is performed. The goal of the this educational module is to familiarize the student physician on the theory behind the lumbar puncture as a diagnostic tool and to guide the student through the steps involved in performing a safe and successful lumbar puncture for the adult patient. Students should complete this module and complete the embedded multiple-choice questions prior to their scheduled lumbar puncture seminar. There will be a brief multiple-choice exam based on this material at the beginning of the seminar.